Effect of Ag2S-BSA nanoparticle size on 3T3 fibroblast cell line cytotoxicity
Ver/
Fecha
2020-03-31Autor
Olivas Armendariz, Imelda
Rodriguez Gonzalez, Claudia
Hernandez Paz, Juan Francisco
Arrieta Sandoval, N
Estrada Rojas, P
Valencia Gómez, Laura Elizabeth
Hernández Cordero, Blanca E.
Metadatos
Mostrar el registro completo del ítemResumen
Silver sulfide nanoparticles, smaller than
5 nm, are of great interest in the biomedical field due
to their improved optical properties from the quantum
confinement of charge carriers. The effect of silver
sulfide nanoparticles (6.4 ± 2.2, 13 ± 4.9, and 33 ±
12.5 nm) coated with BSA (bovine serum albumin) on
cytotoxicity, cell proliferation (MTT assay), and cell
morphology was determined in vitro using 3T3 fibroblast cell line as assay model. The synthesized Ag2SBSA nanoparticles were characterized by X-ray diffraction, infrared spectroscopy, and thermogravimetric analysis. Transmission electron microscopy and dynamic
light scattering were used for particle size measurement.
The MTT results showed a tendency to decrease cell
viability with time exposure rather than concentration,
in all particle’s groups. A greater affectation was observed in cells incubated with smaller nanoparticles, and
this is associated with a higher speed of cell uptake due
to a higher content of adsorbed BSA. It was found that
the largest nanoparticles (33 ± 12.5 nm) at 72 h and at
higher concentration presents the same cell viability as
observed with the smallest attributing it to the agglomeration of particles formed by the low stability of the
sample. Regarding cell morphology, fluorescence
microscopy showed good cell growth in all the nanoparticle groups, observing a slighter increase in the cell
population of the sample of intermediate particle size
(13 ± 4.9 nm).