Evaluation of in vitro bioactivity and in vitro biocompatibility of Polycaprolactone/Hyaluronic Acid/Multiwalled Carbon Nanotubes/Extract from Mimosa tenuilfora composites

Abstract

BACKGROUND:Thedevelopmentofbiomaterialscaffoldsandimplementationoftissueengineeringtechniquesarenecessary. Therefore,Polycaprolactone/SodiumHyaluronate/MultiwalledCarbonNanotubes/ExtractofMimosatenuifloracompositeshave beenproducedbyathermally-inducedphaseseparationmethod. OBJECTIVE: The objective of this research was to evaluate the in vitro bioactivity and in vitro biocompatibility of the composites. METHODS: The in vitro bioactivity of the composites was assessed by soaking them in simulated body fluid for 7, 14, 21, and28days.Thestructureandcompositionofthecompositeswereanalyzedusingscanningelectronmicroscopycoupledwith energydispersivespectroscopyandFouriertransforminfraredspectroscopy.Also,theinvitrobiocompatibilityofthecomposites wasevaluatedbymeansofalkalinephosphataseactivityoftheosteoblastsandbymeasuringthemetabolicactivityofthecells usingMTTassay. RESULTS: The results show a porous and interconnected morphology with enhanced bioactivity. It was observed that the incorporationofMimosatenuiflorainthecompositespromotesincreasedviabilityofosteoblastsinthescaffolds. CONCLUSIONS:Theresultsshowtheefficiencyofbioactiveandbiocompatiblecompositesandtheirpotentialascandidates fortissueengineeringapplications.