EVALUATION OF THE ANTIMICROBIAL EFFECT OF SILVER NANOPARTICLES IN DENTAL PLAQUE ISOLATED FROM PATIENTS WITH ACTIVE CARIES AND ACTIVE PERIODONTAL DISEASE

Abstract

Currently, silver nanoparticles (AgNPs) have been given multiple uses in the area of health has been observed to have a potential as bacteriostatic and bactericidal as has been reported in multiple studies. Dental caries and periodontal diseases are multifactorial infectious diseases which are considered a serious public health problem however, scarce information related to the antimicrobial effect of the AgNPs in dentobacterial plaque has been studied, the objective of the study is to determine the antimicrobial effect and of substantivity of the AgNPs in samples of dental plaque of patients with caries and periodontal disease, Materials and methods Two sizes AgNPs were synthesized and a syringe of consepsis (chlorhexidine) was used. The sample was collected from five patients between the ages of 13 and 60 years. The antimicrobial effect was evaluated through micro dilution tests and at different times in order to see if the antimicrobial effect persisted. This was measured by means of optical density of each sample. Two sizes of AgNPs (5.2nm and 17.5nm) were obtained. Results In the samples collected bacterial inhibition was observed by the AgNPs which presented a concentration of 5.19MG per ML. It was observed that the smaller AgNPs presented a better inhibition compared to the large ones, hours later although the inhibition decreased. they maintained in acéptal ranges, on the other hand chlorhexidine showed better results than AgNPs, the bacteriostatic effect of this substance had a variation in relation to very minimal time. Conclusion The AgNPs showed potential for their clinical application although the effect of chlorhexidine was more effective, it is worth mentioning that the concentrations used were higher than the AgNPs, so their efficacy in different concentrations is still uncertain.