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dc.contributor.authorItza, Mateo Fabian
dc.date.accessioned2018-12-07T17:49:33Z
dc.date.available2018-12-07T17:49:33Z
dc.date.issued2018-01-30
dc.identifier.urihttp://cathi.uacj.mx/20.500.11961/4657
dc.description.abstractBackground: Villi morphology and function affect the absorption capacity of the small intestine. Most tissues are fragile and their morphology may change with excessive manipulation and inadequate sampling techniques. Intestinal sampling includes methodologies such as cutting longitudinally or transversely, keeping the intestinal content in it and preserving all in a 10% formalin solution; washing the intestinal sample in saline solution while emptying it by pressing downwards with two fingers, conserving the sample in a 10% formalin solution and knotting both ends of the sample, introducing 10% formalin into it and preserving it in the same solution. Objective: To compare height, area and desquamation caused by washing, pressing, and knotting used in sampling and conservation techniques of small intestine villi of pigs. Methods: Samples (n = 270) from duodenum, jejunum and ileum of 30 Landrace × Yorkshire crossed pigs, aged 7 to 8 months were randomly subjected to washing, soft pressing or knotting procedures, fixed in 10% formalin solution, embedded in paraffin, and stained with eosin and hematoxylin. Intestinal villi in each slide were observed to determine height, surface area and cellular desquamation of each villus. Results: Villi height from duodenum and ileum knotted samples was higher (p<0.05) compared with samples from the other procedures in the same anatomical portion, which were similar to each other (p>0.05). Villi from knotted jejunum samples were the shortest (p<0.05) compared to the other two procedures, which were similar to each other (p>0.05). Knotted samples from ileum had larger villi area compared with the rest of the procedures and intestinal portions (p<0.05). Villi desquamation was similar among procedures and portions of the intestine (p>0.05). Conclusion: Knotting is the recommended procedure for intestinal cell morphometry evaluation, as values of villi height and area are higher. Desquamation in the three procedures may be related to epithelial restoration processes.es_MX
dc.description.urihttps://aprendeenlinea.udea.edu.co/revistas/index.php/rccp/article/view/328833es_MX
dc.language.isoen_USes_MX
dc.relation.ispartofProducto de investigación ICBes_MX
dc.relation.ispartofInstituto de Ciencias Biomédicases_MX
dc.rightsCC0 1.0 Universal*
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectKeywords: histopathology, knotting, sampling, tissue conservation.es_MX
dc.subject.otherinfo:eu-repo/classification/cti/6es_MX
dc.titleComparison of three sampling procedures for evaluating intestinal villi: A swine model¤es_MX
dc.typeArtículoes_MX
dcterms.thumbnailhttp://ri.uacj.mx/vufind/thumbnails/rupiicb.pnges_MX
dcrupi.institutoInstituto de Ciencias Biomédicases_MX
dcrupi.cosechableSies_MX
dcrupi.norevista1es_MX
dcrupi.volumen3es_MX
dcrupi.nopagina3-9es_MX
dc.identifier.doidoi: 10.17533/udea.rccp.v31n1a01es_MX
dc.contributor.coauthorJaramillo-López, Esaúl
dc.journal.titleRevista Colombiana de Ciencias Pecuariases_MX
dc.lgacNutrición y Comportamiento Reproductivoes_MX
dc.cuerpoacademicoProducción Animales_MX


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