Seventh Biennial Meeting of the North American Society for Comparative Endorinology

Abstract

Introduction: In obesity there is an increase in adipose tissue, which has an endocrine role since it secretes peptides called adipokines, involved in inflammatory and metabolic processes. In this project, the following were studied: resistin, associated with proinflammatory effects in obesity, in this same context chemerin, capable of modulating adipogenesis and finally adiponectin as a biomarker involved in anti-inflammatory processes. Hypothesis: The supplementation with probiotics and prebiotics could modify the genic expression of adipokines associated to the low-grade inflammation of obesity. Main Methods: A murine model (n=50) was used, 5 mice constituted the control group and the remaining 45 were treated with an obesogenic diet for 8 weeks. Afterwards, the control group and 5 obese mice were sacrificed; the rest divided into 4 groups with 4 different diets: 1.- Normocaloric (NC), 2.- NC + probiotic (Lactobacillus acidophillus), 3.- NC + prebiotic (inulin), 4.- NC + symbiotic (L. acidophillus and inulin). A sacrifice is made at 8 weeks of treatment, dissecting the epididymal adipose tissue from which the RNA was extracted for cDNA synthesis. Finally, semiquantitative RT-PCR was performed to evaluate, by means of a densitometric analysis, the relative expression index (REI) of the genes of interest, using the gen 18sRNA as constitutive. Results: Supplementation for 8 weeks triggers repression of adipokine genes related to obesity and inflammation. The better results were obtained when the animal model was supplemented with the NC + synbiotic diet, this repression was specifically observed in chemerin: it starts with a REI of 0.722 (±0.951) in the model with obesity and is repressed to an REI of 0.488 (± 0.713), also in resistin, REI where the value was modified from 1.980 (±1.976) to 1.064 (± 1.128). On the other hand, in adiponectin an increase in expression was observed, obtaining an REI of 0.134 (±0.100) to an IER of 0.912 (±0.891). Conclusion: A synbiotic composed of L. acidophillus and inulin, can be postulated as a viable additive for modifying the expression of genes that promote a negative regulation of the development inflammation associated with obesity, thus we can consider synbiotics an complementary anti obesity therapy. (Our gratitude to the support given from CONACYT, with the grant 1080026)