Comparison between Allura Red dye discoloration by activated carbon and azo bacteria strain

Abstract

Azo dyes are extensively used in different industries areas, such as Allura Red (R-40). Previous studies have proven its carcinogenic and mutagenic properties. For the removal of this type of emerging pollutant from effluents, tertiary treatment techniques such as activated charcoal are used. Alternatively, the use of bacteria is preferred because of its quick discoloration processes. The aim of the present investigation is to compare the efficiency removal of R-40 from aqueous media by a physicochemical process and a biological one. The sorption kinetics of 10 ppm of R-40 was carried out with the use of activated charcoal based on walnut shells in water. Moreover, Pseudomonas aeruginosa and Bacillus subtilis stains were used separately to decolorize nutrient broth media supplementedwith 50 ppmof R-40. The activated carbon was capable to remove 99.87% of R-40 at 264 h, while the bacterial strains decolorized 92.13% (P. aeruginosa) and 88.21% (B. subtilis), respectively, under microaerophilic conditions after 168 h. Therefore, both process strategies, physicochemical and biological rapprochements, were able to remove the dye from aqueous media. R-40 was not cytotoxic to used strains, besides gram-positive either negative bacteria could be applied to turn over this azo dye in short term. Combination of both approaches may be implemented in tandem architecture.